Microscopy Core Facilities

Overview

Calendars

Click on a link to view a schedule.

Microscopy Core Facilities (MCF) is a center at Wright State University that offers equipment support in imaging and analysis. The center is open to research-engaged faculty.

Use of the facility will be determined by the director. All users must be trained and approved by core personnel before they will be able to use equipment unsupervised. Scheduling time for equipment will be done via an online calendar.

iLab Registration

Images from the confocal and electron microscopes

Equipment

Confocal Microscopes

All microscopes are upright and all set up for DIC-IR illumination.

Olympus FV1000

photo of the olympus fv1000

Six excitation lines (405, 488, 458, 511, 568, 633) and four fluorescence detection lines (two spectral units and two based on optical filters) and one detector for transmitted bright field. (New in October 2006. Current fee $20/hour.)

Olympus FV300

photo of the olympus fv300Two excitation lines (488, 568) and two detectors that can be switched for different combinations of dual fluorescence or fluorescence and transmitted light simultaneous detection. (Major upgrade in November 2007. Current fee $20/hour.)

Olympus 2-photon

photo of the olympus 2 photonTwo-photon excitation is obtained with a MaiTai pulsed laser (range 700-1040 nm). Single photon excitation is also possible (488, 458, 511, 559, 633 lines). Detection can be accomplished through four non-descan detectors, three confocal detectors and one transmitted light detector. (New in September 2009. Use of this instrument and fees need to be agreed by the "2-photon user group.")

MCF is not responsible for any data saved on the network.


Electron Microscope

photo of the electron microscopeThe Phillips 208S is a 100kv transmission electron microscope with excellent contrast and resolution with properly prepared specimens. The instrument, besides producing data-rich film output, is also coupled to AMT xr611 camera that permits high-resolution digital imaging.

The core provides technical help for the use of the microscope (inserting and withdrawing specimens, processing any EM negatives). Thus, usage of this instrument is always implemented with assistance by authorized personnel (Jackie Sisco or Ricardo Zerda). The fee is $20/hour with technical assistance. EM negative usage will be charged separately depending on the level of consumption.

The core does not have enough personnel resources to help with specimen preparation.


Histocore

The Histocore is a facility designed to provide equipment support for research faculty in tissue sectioning and staining.

Equipment
  • HM 550 Cryostat
  • HM505 E Cryostat
  • Freezing Sliding Microtome
  • Vibratome
  • Ultramicrotome MT 5000
  • Ultramicrotome MT 6000
  • Olympus Epi Fluorescence Spot Scope with RT color camera

Use of the facility will be determined by the director. All users have to be trained and approved by core personnel before being able to use equipment unsupervised.

MCF only provides equipment and training on use of the equipment. Tissue preparations are to be done by the principal investigator's lab.


Review Stations

The MCF provides three network review stations used for imaging processing and analysis:

  • Review I: Fluoview (FV300), Image Pro 5.1 with 3-d reconstruction, Corel Draw 12, and Office Suite
  • Review II: Fluoview 1.7, Image Pro 7.0, Corel Draw 12 and Office Suite
  • Review III (Monica): Image Pro 5.1, Photoshop 7.0, Corel Draw 12, and Office Suite
  • Other Hardware: Xerox Phaser 6300 Color Laser Printer, HP 1500n Monochrome Laser printer, and Imacon Flextight 848 Digital negative scanner (Review III).

The MCF is not responsible for any data saved on the network.

Policies & Fees

Policies

Usage of the core will be determined by the director and/or the assistant directors.

Training will be completed by the director and/or the assistant directors.

Fees

Equipment

New Rates

2 photon

$20/hour

Fv1000 (four lasers: 405,488,568,647)

$15/hour

Fv300 (two lasers: 488, 568)

$10/hour

Electron Microscope—TEM

$15/hour

Microscope monthly usage fee (includes fluorescent upright and fluorescent dissecting scope)

$10/month

Neurolucida/Stereoinvestigator/Huygens

$20/month

Review Station monthly fee

$20/month

Histocore monthly usage fee

$10/month

Perfusion lab

$10/month

One major microscope one year unlimited

$6,000/ year

Microscope unlimited fee: confocals, EM (2 photon not included)

$7,000/year

Training Fee

$20/hour

Note: Special consideration will be given to PPG group members and P.I.'s who include in their grant budgets support (full or partial) for the extended warranties, maintenance plans or major upgrades of the core instrumentation.

Last edited on 06/04/2015.